Bovine Brucellosis Antibody ELISA Kit

The Brucella Antibody Competitive ELISA Kit is based on competitive ELISA, to detect brucella antibody in serum, plasma samples, which is rapid, accurate and easy-to-operate. The test time is 60min. The target brucella strains include B. abortus, B. melitensis, and B. ovis, etc

Background

Brucellosis, also known as undulant fever, Mediterranean fever is a zoonosis and the infection is almost invariably transmitted by direct or indirect contact with infected animals or their products. Expansion of animal industries and urbanization, and the lack of hygienic measures in animal husbandry and in food handling partly account for brucellosis remaining a public health hazard. Expansion of international travel which stimulates the taste for exotic dairy goods such as fresh cheeses which may be contaminated, and the importation of such foods into Brucella-free regions, also contribute to the ever-increasing concern over human brucellosis. 

Introduction of Brucella Antibody Competitive ELISA Kit

The brucella antibody ELISA kit is based on competitive ELISA to measure the amount of brucella specific antibodies in bovine/goat/sheep/swine serum. In the kit, brucella LPS antigen is pre-coated in microplates. During the assay, samples are added to the microplate wells, brucella specific blocking antibody is also added. If brucella specific antibody is present in sample, it will compete with the blocking antibody for coated LPS antigen on the wells and forms an antibody-antigen complex. Unbonded antibody will be removed with a wash step. Later HRP conjugated is added to combine with the brucella specific antibodies captured in the wells. Wash again to remove free HRP conjugate, then substrate is added to show the color. The color of the wells is inversely correlated to the concentration of the brucella specific antibodies in the sample.

The Brucella Antibody Competitive ELISA Kit is based on competitive ELISA, to detect brucella antibody in serum, plasma samples, which is rapid, accurate and easy-to-operate.

Application of this Brucella Antibody Competitive ELISA Kit

Serum, blood, plasma and milk of cattle, sheep, goat, swine, the target brucells species include Brucella abortus, Brucella melitensis, and Brucella suis, Brucella ovis

Technical Details of this Brucella Antibody Competitive ELISA Kit

Note: this kit does not contain any contagious objects, it is safe to use. Only samples shall be treated carefully.

Brucella Antibody Competitive ELISA Kit Components

This Brucella Antibody Competitive ELISA Kit is available in the format of 96wells, 192wells and 480wells. The component of each format is different. Please always check the kit instructions for details.

• Precoated microtiter plate, 96wells
• Sample buffer, 1 vial
• Positive Control, 1 vial
• Negative Control, 1 vial
• Enzyme Conjugate, 1 vial
• TMB substrate, 1 vial
• Stop solution, 1 vial
• Kit instruction, 1 set

Citations

• Jhandai, P., Shanmugasundaram, K., Gambhir, D., Nale, T., Shewale, A., Tiwari, S., & Singha, H. (2023). Diagnosis of Various Bacterial Zoonotic Diseases in Human and Bovine populations in Haryana State under the umbrella of One Health. Indian Journal of Veterinary Public Health, 9(3), 26.
• Patel, B. K., Pandey, A., Chandel, B. S., & Chauhan, H. C. (2022). EVALUATING SEROLOGICAL AND MOLECULAR METHODS USED FOR THE DETECTION OF BRUCELLA FROM CATTLE AND BUFFALO.

References

• Nielsen, K. H., Wright, P. F., Kelly, W. A., & Cherwonogrodzky, J. H. (1988). A review of enzyme immunoassay for detection of antibody to Brucella abortus in cattle. Veterinary immunology and immunopathology, 18(4), 331-347.
• Nielsen, K., Smith, P., Gall, D., Perez, B., Cosma, C., Mueller, P., ... & Bosse, J. (1996). Development and validation of an indirect enzyme immunoassay for detection of antibody to Brucella abortus in milk. Veterinary Microbiology, 52(1-2), 165-173.