Rotavirus Real-time PCR Kit

Porcine Rotavirus Real-time PCR Kit uses Taqman fluorescent probes to amplify and detect the porcine rotavirus (PoRV) gene, which can be applied to porcine rotavirus (PoRV) nucleic acid detection in tissues such as jejunum and small intestine, stool samples, and blood samples

Rotavirus (RV) is considered a major cause of acute viral gastroenteritis in young animals. RV is classified into nine species, five of which have been identified in pigs. Most studies worldwide have highlighted diarrhoea outbreaks caused by RVA, which is considered the most important RV species

Rotaviruses are ubiquitous and they are present in most if not all pig herds with virtually a 100% sero-conversion in adult stock. A further epidemiological feature is their persistence outside the pig, where they are resistant to environmental changes and many disinfectants. Maternal antibodies persist for 3-6 weeks after which pigs become susceptible to infection but exposure does not necessarily result in disease. It is estimated that only 10-15% of diarrhoeas in pigs are initiated by a primary rotavirus infection. The fact that the virus persists in the environment accounts for widespread infection and therefore a constant risk of disease.

Key facts of this Porcine Rotavirus Real-time PCR Kit

• Ready to use kits for veterinary labs
• No special instrument required
• Only less than 60min to get the result
• Low cost with high sensitivity

Performance of the Porcine Rotavirus Real-time PCR Kit

This kit uses Taqman fluorescent probes to amplify and detect the porcine rotavirus (PoRV) gene, which can be applied to porcine rotavirus (PoRV) nucleic acid detection in tissues such as jejunum and small intestine, stool samples, and blood samples

Porcine Rotavirus Real-time PCR Kit Components

• Negative Control
• Positive Control
• Enzyme Mix
• Sample buffer

Extended Reading

• The Pig Site, Rotavirus page
• MSD Veterinary Manuals, Rotavirus