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African Swine Fever AgRapid Test Kit

African Swine Fever AgRapid Test Kit

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R 186.20 186.2 ZAR R 186.20 Tax Excluded

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    Animal Tests Swine / Porcine

    Need a quote, bulk pricing or technical support? Contact RingbioSA on 076 608 3730 or email info@ringbiosa.co.za. Product instructions and certificates, where available, can be downloaded in the Documents section above.

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    African Swine Fever Virus antigen ASFV Ag Rapid Test Kit is used to detect ASF antigen in swine serum, plasma, and whole blood.

    Basic information

    African swine fever (ASF) is a severe viral disease affecting domestic and wild pigs. More recently (since 2007) the disease has been reported in multiple countries across Africa, Asia, and Europe, in both domestic and wild pigs. In 2018 and 2019, ASFV is spreading quickly to Asia, Southeast Asia. In countries like China, Vietnam, etc now ASFV is becoming a big threat to local animal farming.

    This ASFV antigen rapid test kit is based on lateral flow immunoassay to detect antigen of ASFV in swine serum/plasma/tissue extract, etc. which only takes 10-15min to get a visible result. No special instrument required and no extra cost added, which is quite suitable for lab tests and field tests.

    The antibodies used in this kit is a paired monoclonal antibody against ASFV p72, P30 and P54 protein. Sensitivity is also enhanced by mixed other ASFV specific antibodies.

    Note: this kit does not contain any contagious materials, which is not hazardous to human or animal.

    Product nameASFV Ag Rapid Test Kit
    Product codeC30021
    PrincipleSandwich antibody immunoassay
    SpeciesSwine, porcine
    Specificy>95%
    Sensitivity>90% compared with Real-time qPCR when testing the infected dead pigs.
    Detection limit106 - 107 TCID50/ml, Ct value of qPCR is approx. 25 according to validation by EU Lab
    Shelf-life12 months
    Storage2- 30 oC or 2-8 oC

    * The detection limit of this kit was tested by the ASF Reference Laboratory of Canada

    Key facts of the African Swine Fever Virus antigen ASFV Ag Rapid Test Kit

    • Ready to use kits for farmers and the general lab staff
    • No special instrument required
    • Suitable for field test
    • Result in 10-15min
    • Detection limit: this kit detects ASF Ag to CT value of 30 or less according to comparison research done by our customers.

    African Swine Fever Virus antigen ASFV Ag Rapid Test Kit Components

    ASFV Ag Test Components
    Item #ItemQty
    1ASFV Ag Rapid Test Card 50 pcs
    2Sample buffer, 5ml per bottle 3 bottles
    3Plastic pipettes, 0.5ml 50 pcs
    4Kit instruction,  1 set.

    Video on how to operate the ASFV Ag Rapid Test Kit

    Certificates

    • This kit has been submitted for registration in the China Ministry of Agriculture and Rural Affairs

    Citations

    1. Jarynowski, A., Płatek, D., Krzowski, Ł., Gerylovich, A., & Belik, V. (2019). African Swine Fever-potential biological warfare threat (No. 1904). EasyChair.
    2. Cavalera, Simone, et al. "Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues." Talanta 258 (2023): 124443.
    3. HA, Angi, and R. D. Tulle. "Seroprevalence Rate and Factors Causing African swine fever Outbreak in Kupang City and Regency of East Nusa Tenggara Province, Indonesia." (2023).
    4. Cavalera, Simone, et al. "Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues." Talanta 258 (2023): 124443.

    Extensive reading