Newcastle disease virus Real-time PCR kit
Need a quote, bulk pricing or technical support? Contact RingbioSA on 076 608 3730 or email info@ringbiosa.co.za. Product instructions and certificates, where available, can be downloaded in the Documents section above.
About Newcastle Disease (NDV / APMV-1)
- What it is: endemic viral disease — single biggest commercial poultry disease risk in SA.
- OIE/WOAH status: notifiable.
- SA context: vaccination is routine; antibody titre monitoring confirms vaccine take; outbreak diagnosis uses PCR.
- Typical samples: cloacal + oropharyngeal swabs (PCR), serum (titre).
- Who cares in SA: commercial poultry, backyard flocks, state vets, poultry vets.
Newcastle disease (ND) is a highly contagious and often severe disease found worldwide that affects birds including domestic poultry. It is caused by virulent strains of avian paramyxovirus type 1. The disease appears in three forms: lentogenic or mild, mesogenic or moderate and velogenic or very virulent, also called Newcastle disease. The lentogenic strains are very widespread but cause few disease outbreaks. It usually presents as a respiratory disease, but depression, nervous manifestations, or diarrhoea may be the predominant clinical form. This Newcastle disease virus Real-time RT-PCR kit is used to detect Newcastle disease virus.
Key facts of Newcastle disease virus Real-time RT-PCR kit
- No toxic organic solvent required.
- High recovery of nucleic acid.
- Extraction in 30min.
Newcastle disease virus Real-time RT-PCR Kit Components
| Item # | Item | Qty |
|---|---|---|
| 1 | NDV reaction solution | 700ul |
| 2 | NDV enzymes | 50ul |
| 3 | Positive control | 100ul |
| 4 | Negative control | 100ul |
Prevention and control
When the disease appears in a previously disease free area, a stamping out policy is practiced in most countries. This includes:
- strict isolation or quarantine of outbreaks
- humane destruction of all infected and exposed birds （WOAH Terrestrial Animal Health Code）
- thorough cleaning and disinfection of premises
- proper carcass disposal （WOAH Terrestrial Animal Health Code）
- pest control in flocks
- depopulation followed by 21 days without poultry before restocking
- avoidance of contact with birds of unknown health status
- control of access to poultry farms.
Further reading
WOAH, newcastle disease