Zearalenone ZON/F2-toxin ELISA Kit
Need a quote, bulk pricing or technical support? Contact RingbioSA on 076 608 3730 or email info@ringbiosa.co.za. Product instructions and certificates, where available, can be downloaded in the Documents section above.
Documents
About Zearalenone (ZON / F-2 toxin)
- What it is: a Fusarium graminearum mycotoxin with oestrogenic activity.
- Health status: endocrine disruptor — causes reproductive disorders in pigs and dairy cattle; concern for growing pigs especially.
- EU guidelines (feed): typically 100 µg/kg for piglet feed, 500 µg/kg for sow feed, up to 3 000 µg/kg for adult cattle feed.
- Where it's found: maize, wheat, barley; particularly in cool, humid grain seasons.
- Typical samples: grain intake, compound feed, finished-feed release.
- Who cares in SA: pig producers (reproductive-health focus), feed mills, maize buyers.
Zearalenone ELISA Kit is a competitive ELISA kit to detect zearalenone in feed and cereal samples.
Zearalenone (ZEN, ZEA, or ZON), also known as RAL and F-2 mycotoxin, is a potent estrogenic metabolite produced by some Fusarium and Gibberella species. Particularly, is produced by Fusarium graminearum, Fusarium culmorum, Fusarium cerealis, Fusarium equiseti, Fusarium verticillioides, and Fusarium incarnatum.
This Zearalenone ELISA Kit is based on indirect competitive ELISA, the coupled antigen was pre-coated in the microwells, the zearalenone will compete for the antibody with the pre-coated antigen, then add HRP enzyme conjugate and TMB substrate successively, the OD value of the sample is negatively correlated with the content of zearalenone contained in the sample. The standard curve is fitted by regression and multiplied by the corresponding dilution ratio to obtain the content of zearalenone in the sample.
Key facts of the Zearalenone ELISA Kit
- Ready to use ELISA kit based on Competitive Immunoassay
- Total time required only 75min
- Highly specific to zearalenone
- Applicable for both feed and cereal (including maize, feedstuff, etc)
Zearalenone ELISA Kit parameters
- Sensitivity: 0.1 ng/ml (ppb)
- Stability: 12 months when stored properly
- Accuracy: 80%-120%
Zearalenone ELISA Kit components
- Precoated microwell, 96wells
- Zearalenone standard series, 0.1 - 8.1 ng/ml
- Enzyme conjugate
- TMB Substrate
- Stop solution (not available when shipping by air)
- Sample buffer
- Wash buffer
Zearalenone ELISA Kit related products
- Aflatoxin B1 Rapid Test Kit for feed
- Aflatoxin B1 ELISA Kitfor feed
For more information about the Zearalenone ELISA kit, please feel free to contact us.
Further readings on aflatoxins and aflatoxin testing:
- Caldwell, Rodney W., et al. "Zearalenone production by Fusarium species." Applied microbiology 20.1 (1970): 31-34.
- De Saeger, Sarah, Liberty Sibanda, and Carlos Van Peteghem. "Analysis of zearalenone and α-zearalenol in animal feed using high-performance liquid chromatography." Analytica Chimica Acta 487.2 (2003): 137-143.