Deoxynivalenol (Vomitoxin) DON ELISA Kit
Need a quote, bulk pricing or technical support? Contact RingbioSA on 076 608 3730 or email info@ringbiosa.co.za. Product instructions and certificates, where available, can be downloaded in the Documents section above.
Documents
About Deoxynivalenol (DON / Vomitoxin)
- What it is: a Fusarium graminearum trichothecene mycotoxin common on cereals grown under cool, wet conditions.
- Health status: causes feed refusal, vomiting, and immunosuppression — particularly in swine; cattle are less sensitive.
- EU limit (unprocessed cereals): 1 250 µg/kg for food use; feed guidance 900–8 000 µg/kg depending on species.
- Where it's found: wheat, barley, maize — common in SA Eastern Cape and KZN maize belt in wet seasons.
- Typical samples: wheat intake at mills, maize grain, compound feed.
- Who cares in SA: swine producers, wheat and maize millers, breweries.
Deoxynivalenol ELISA Kit is a competitive ELISA kit to detect deoxynivalenol (vomitoxin, DON) in feed and cereal samples.
Vomitoxin, also known as deoxynivalenol, is a type B trichothecene, an epoxy-sesquiterpenoid. This mycotoxin occurs predominantly in grains such as wheat, barley, oats, rye, and corn, and less often in rice, sorghum, and triticale.
This Deoxynivalenol (vomitoxin, DON) ELISA Kit is based on indirect competitive ELISA, the coupled antigen was pre-coated in the microwells, the zearalenone will compete for the antibody with the pre-coated antigen, then add HRP enzyme conjugate and TMB substrate successively, the OD value of the sample is negatively correlated with the content of zearalenone contained in the sample. The standard curve is fitted by regression and multiplied by the corresponding dilution ratio to obtain the content of zearalenone in the sample.
Key facts of the Deoxynivalenol ELISA Kit
- Ready to use ELISA kit based on Competitive Immunoassay
- Total time required only 75min
- Highly specific to Deoxynivalenol (vomitoxin, DON)
- Applicable for both feed and cereal (including maize, feedstuff, etc)
Deoxynivalenol ELISA Kit parameters
- Sensitivity: 0.1 ng/ml (ppb)
- Stability: 12 months when stored properly
- Accuracy: 80%-120%
Deoxynivalenol ELISA Kit components
- Precoated microwell, 96wells
- Deoxynivalenol (vomitoxin, DON) standard series, 0.1 - 8.1 ng/ml
- Enzyme conjugate
- TMB Substrate
- Stop solution (not available when shipping by air)
- Sample buffer
- Wash buffer
Deoxynivalenol ELISA Kit related products
- Aflatoxin B1 Rapid Test Kit for feed
- Aflatoxin B1 ELISA Kitfor feed
- Zearalenone ELISA Kitfor feed
For more information about the Deoxynivalenol (vomitoxin, DON) ELISA Kit, please feel free to contact us.
Further readings on aflatoxins and aflatoxin testing:
- Cote, L. M., et al. "Survey of vomitoxin-contaminated feed grains in midwestern United States, and associated health problems in swine." (1984).
- Lun, A. K., L. G. Young, and J. H. Lumsden. "The effects of vomitoxin and feed intake on the performance and blood characteristics of young pigs." Journal of animal science 61.5 (1985): 1178-1185.
- Casale, William L., James J. Pestka, and L. Patrick Hart. "Enzyme-linked immunosorbent assay employing monoclonal antibody specific for deoxynivalenol (vomitoxin) and several analogs." Journal of Agricultural and Food Chemistry 36.3 (1988): 663-668.